21-Hydroxylase Antibody - RIA

RA007/50

125I-Radioassay for the quantitative determination
of IgG autoantibodies against 21-Hydroxylase in serum
 

2 x 25 determinations
2 controls

20 µl of sample
separation with solid phase Protein A
6 standards, 2 controls, ready for use
incubation over-night

Endocrinology, Immunology

Addison's disease, isolated or part of the autoimmune polyglandular syndromes (APS) type I or type II

<1 U/ml

98 % versus normals and other autoimmune diseases

97 % for Addison's disease in APS type I or II
72 % for isolated Addison's disease

125I-labelled highly purified recombinant 21-Hydroxylase
superior to immunofluorescence:
  higher specificity
  detection of antibodies to the only relevant target antigen for Addison's disease
  less equivocal results