Proteinase 3 Antibody (C-ANCA) - ELISA

EA004/96

Enzyme immunoassay for detection and quantification of
proteinase 3 autoantibodies in serum
 

12 x 8 single break apart well microtiter strips (96 determinations)
 

Internal medicine (Nephrology, Rheumatology, Immunology, Pulmonology), Oto-rhino-laryngology, Ophthalmology

Systemic diseases, especially primary vasculitides, e.g. Wegener's granulomatosis, microscopic polyangiitis, panarteritis nodosa, Churg-Strauss-Syndrome

98 % for Wegener's granulomatosis
 

40 - 93 % depending on disease activity and extent
  *Nölle et al., Ann. Intern. Med. 111 (1989) 28-40*
 

very specific for Wegener's granulomatosis due to the use of highly purified proteinase 3, the target antigen of C-ANCA. 
Due to impure antigen-preparations (granule extracts) used by other manufacturers, several false-positive results can be obtained with their ELISAs, e.g. in patients with other autoimmune disorders like SLE, Goodpasture's syndrome or Henoch-Schoenlein purpura. Moreover, some patients with high concentrations of myeloperoxidase antibodies can be found positive in these ELISAs *Rasmussen et al., J. Immunol. Methods 712 (1990) 139-145*
  * no subjective criteria as in indirect immunofluorescence
  * follow-up control of disease activity by quantitative results
  * parallel processing with the Anti-MPO-ELISA for detection of myeloperoxidase autoantibodies is possible; many of the reagents can be used from either of the kits