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anti-IA2 - ELISA |
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Catalog-No. |
EA105/96 Enzyme Immuno Assay for the Quantitative Determination of Antibodies against IA-2 in Serum |
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Format |
12 x 8 determinations |
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Procedure |
The IA-2 Antibody ELISA depends on the ability of IA-2 autoantibodies to act divalently and form a bridge between IA-2 coated on ELISA plate wells and liquid phase IA-2-biotin. The IA-2-biotin bound is is then quantitated by addition of streptavidin peroxidase and a colorogenic substrate (TMB) with reading of final absorbance at 2 wavelengths (450 and 405 nm) to obtain maximum measuring range (7.5 - 4,000 units per ml of WHO reference preparation NIBSC 97/550). |
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Clinical Fields |
Internal medicine, immunology, endocrinology |
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Diseases |
Insulin Dependent Diabetes Mellitus (IDDM) |
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Normal Value |
Healthy blood donor sera give values of less than 7.5 Units per ml (NIBSC 97/550) in the assay suggesting that values of 7.5 U/ml or greater can be considered positive for IA-2 autoantibodies. However, individual laboratories should establish their own cut off values using appropriate control sera. |
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Specificity |
Results from the DASP 2005 study indicate 99% specificity (n=100).. |
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Sensitivity |
Results from the DASP 2005 study indicate 66% sensitivity (n=50). |
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Instruction Sheet |
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