IA2 Antibody - ELISA
Enzyme Immuno Assay
for the quantitative determination of
antibodies against IA-2 in serum
The IA-2 Antibody ELISA depends on the ability of IA-2 autoantibodies to act divalently and form a bridge between IA-2 coated on ELISA plate wells and liquid phase IA-2-biotin. The IA-2-biotin bound is is then quantitated by addition of streptavidin peroxidase and a colorogenic substrate (TMB) with reading of final absorbance at 2 wavelengths (450 and 405 nm) to obtain maximum measuring range (7.5 - 4,000 units per ml of WHO reference preparation NIBSC 97/550).
Internal medicine, immunology, endocrinology
Insulin Dependent Diabetes Mellitus (IDDM)
Healthy blood donor sera give values of less than 7.5 Units per ml (NIBSC 97/550) in the assay suggesting that values of 7.5 U/ml or greater can be considered positive for IA-2 autoantibodies. However, individual laboratories should establish their own cut off values using appropriate control sera.
Results from the DASP 2005 study indicate 99% specificity (n=100)..
Results from the DASP 2005 study indicate 66% sensitivity (n=50).