Product Information

IA2 Antibody - ELISA

Catalog-No.

EA105/96
Enzyme Immuno Assay
for the quantitative determination of
antibodies against IA-2 in serum

Format

12x8 Determinations

Procedure

The IA-2 Antibody ELISA depends on the ability of IA-2 autoantibodies to act divalently and form a bridge between IA-2 coated on ELISA plate wells and liquid phase IA-2-biotin. The IA-2-biotin bound is is then quantitated by addition of streptavidin peroxidase and a colorogenic substrate (TMB) with reading of final absorbance at 2 wavelengths (450 and 405 nm) to obtain maximum measuring range (7.5 - 4,000 units per ml of WHO reference preparation NIBSC 97/550).

Clinical Fields

Internal medicine, immunology, endocrinology

Diseases

Insulin Dependent Diabetes Mellitus (IDDM)

Normal Value

Healthy blood donor sera give values of less than 7.5 Units per ml (NIBSC 97/550) in the assay suggesting that values of 7.5 U/ml or greater can be considered positive for IA-2 autoantibodies. However, individual laboratories should establish their own cut off values using appropriate control sera.

Specificity

Results from the DASP 2005 study indicate 99% specificity (n=100)..

Sensitivity

Results from the DASP 2005 study indicate 66% sensitivity (n=50).