CAT (AD/NAD/DA) - ELISA
Enzyme Immuno Assay
for the quantitative determination of
Adrenaline / Noradrenaline / Dopamine in Plasma and Urine
The assay kit provides materials for the quantitative measurement of adrenaline, noradrenaline and dopamine in plasma and urine; 3 x 96 determinations.
Noradrenaline, adrenaline and dopamine are extracted using a cis-diol-specific affinity gel and acylated to N-acylnoradrenaline, N-acyladrenaline and N-acyl-dopamine and then converted enzymatically into N-acylnormeta¬nephrine, N-acylmetanephrine and N-acyl-3-methoxytyramine.
The competitive CAT ELISA kit uses the microtitre plate format. Adrenaline, noradrenaline and dopamine, respectively, are bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites.
Endocrinology, Oncology, Psychiatry
Pheochromocytoma, neuroblastoma, ganglioneuroma, degenerative cardiac diseases, hyper- and hypotension, schizophrenia, manic-depressive psychosis, stress, burn-out syndrome
The reference ranges given below should only be taken as a guideline. It is recommended that each laboratory should establish its own normal values.
Adrenaline Noradrenaline Dopamine
Urine < 20 µg/day < 90 µg/day < 600 µg/day
Plasma < 100 pg/ml < 600 pg/ml < 100 pg/ml
see instruction for use
The lower limit of detection was determined by taking the 2fold standard deviation of the absorbance of the Zero Reference and reading the corresponding value from the standard curve.
Adrenaline Noradenaline Dopamine
Sensitivity (Urine): 0.08 ng/ml 0.24 ng/ml 0.44 ng/ml
Sensitivity (Plasma): 5 pg/ml 16 pg/ml 29 pg/ml