Product Information
1-Methylhistamine (N-Methylhistamine) - ELISA
EA208/96
Enzyme-linked Immuno Assay for the quantitative determination of
1-Methylhistamine (N-Methylhistamine) in human urine
Competitive ELISA
96 Determinations
Microtiter plate with 12 single break apart 8 well strips
Storage at 2-8°C, Transport at ambient temperature
6 standards, ready for use: 0 (0); 10 (80); 30 (240); 100 (800); 300 (2400); 1000 (8000) ng/ml (nmol/l)
2 positive controls, ready for use
Sample volume: 20µl urine
1. Dilution and Acylation of samples, standards and controls (incubation time 5 and 20min)
2. ELISA (incubation times 30, 20 and 25min)
3. Read at 450nm (570-650nm reference) within 15min
Allergology, Pharmacology
see Instructions for Use
Urine, 24h < 250 μg/day
Urine, spontaneous 30 - 200 μg/g Creatinine
| Substance | Cross Reactivity [%] |
| 1-Methylhistamine | 100 |
| Histamine | <0.7 |
| 3-Methylhistamine | <0.007 |
| 1-Methyl-4-imidazole-acetic acid | <0.0025 |
| Imidazole-4-acetic acid | <0.007 |
| L-Histidine | <0.0025 |
Lower Detection Limit: 3.0 ng/ml urine
Correlation LC/MS R=0.993 (n=32)
Extended standard range enables the measurement of healthy and pathological
samples without pre-dilution
Shelf life of up to 24 months
No known interference with drugs
Highly sensitive and specific antibodies with very low cross-reactivity
Produced in DIN EN ISO 13485-certified facility in Germany
EU: CE-certified IVD; non-EU: RUO