Product Information

Zinc Transporter 8 (ZnT8) Antibody - ELISA

Catalog-No.

EA102/96
Enzyme Immuno Assay
for the quantitative determination of autoantibodies to the
zinc transporter 8 (ZnT8) in serum

Format

12x8 Determinations
micotiter strips, single break apart

Procedure

25 microlitre calibrators, controls and samples into wells,

18 hrs. incubation, 3 x wash

add ZnT8 biotin, 1h incubation

3 x wash, add SAPOD, 20 min incubation

3 x wash, add substrate. 20 min incubation

stop reaction + read

Clinical Fields

Internal medicine, immunology, endocrinology

Diseases

Type 1 Diabetes Mellitus (DM)

Normal Value

Negative < 15 U/ml; Positive > 15 U/ml

Specificity

97 % ; n = 90 for healthy blood donors (DASP 2015)

Sensitivity

76 %; n = 50 for type 1 DM patients (DASP 2015)

Characteristics

Reliable and convenient method to measure specific ZnT8 antibodies which are major component of ICA in type 1 DM and wihich are useful for diagnosis and prediction of type 1 DM. The non-isotopic assy is suitable in routine clinical laboratories in automated or manual formats.

Literature:

J. M. Wenzlau et al.
The cation efflux transporter ZnT8 (Slc30A8) is a major autoantigen in human type I diabetes.
PNAS 2007 104:17040-17045

P. Achenbach et al.
Autoantibodies to zinc transporter 8 and SLc30A8 genotype stratify type 1 diabetes risk.
Diabetologia 2009 52:1881-1888

J. M. Wenzlau et al .
Kinetics of the post-onset decline in zinc transporter 8 autoantibodies in type 1 diabetic human subjects.
J Clin Endocrinol Metab 2010 95:4712 - 4719

G. Dunseath et al
Bridging-type enzyme-linked immunoassay for zinc transporter 8 autoantibody measurements in adult patients with diabetes mellitus.
Clin. Chim. Acta. 2015 447:90 - 95

 

 

Instruction Sheet