Product Information

Calretinin - ELISA

Catalog-No.

EA611/96
Enzyme-linked Immuno Assay for the quantitative determination of
Calretinin in human EDTA-plasma and serum


Format

Sandwich ELISA
96 Determinations
Microtiter plate with 12 single break apart 8 well strips,
coated with polyclonal calretinin antibodies
Storage at 2-8°C, Transport at ambient temperature
6 standards: 0; 0.25; 0.5; 1; 2; 4 ng/ml
2 positive controls


Procedure

Sample volume: 15 µl patient serum or EDTA-plasma

1. Preparation of samples, standards and controls (incubation time 60min)
2. ELISA (incubation times 120, 60, 60 and 30min)
3. Read at 450nm (570-650nm reference) within 15min


Clinical Fields

Oncology, cancer screening, occupational diseases


Diseases

Mesothelioma


Normal Value

Men: <0.6 ng/ml
Women: <0.8 ng/ml


Specificity

n. d.


Sensitivity

Lower Detection Limit: 0.05 ng/ml


Characteristics

Highly specific and sensitive rabbit anti-calretinin antibody
Good linearity between 0.25 - 4.0 ng/ml
Validated in larger case-control studies
Involved in prospective studies

Produced in DIN EN ISO 13485-certified facility in Germany

EU: CE-certified IVD; non-EU: RUO


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Instruction Sheet