Product Information

Calretinin - ELISA

Catalog-No.

EA611/96
Enzyme Immuno Assay

Format

12x8 Determinations,
Microtiter plate, single break apart wells; coated with polyclonal calretinin antibodies

Procedure

Sample volume: 15 µl patient serum or plasma

1. Sample Preparation: 60 min shaking at room temperature

2. ELISA: 5 hours incubation at room temperature

Measurement in ELISA reader at 450 nm (620 nm reference wavelength)

6 Standards with range: 0.25 ng/ml - 4.0 ng/ml

2 Kit controls, lyophilized

Enzyme Conjugate 200 x concentrated; Wash buffer 50 x concentrated

All other reagents ready for use

Clinical Fields

Oncology, cancer screening, occupational diseases

Diseases

Mesothelioma

Normal Value

Specificity

see instruction for use

Sensitivity

0.05 ng/ml

Characteristics

Sandwich ELISA with microtiter plate formate

Highly specific and sensitive rabbit anti-calretinin antibody

Good linearity between 0.25 - 4.0 ng/ml

Easy to handle, total assay time is 6 hours

Suitable for testing large series of serum or plasma samples

Validated in larger case-control studies

Involved in prospective studies

Instruction Sheet