Product Information
ACHRAB Assay - ELISA
EA110/96
Enzyme Immuno Assay
or the Quantitative Determination of
Acetylcholine Receptor Autoantibodies in Serum
12x8 Determinations,
Microtiter plate, single break apart wells; coated with monoclonal antibody MAb1
4 Standards with range: 0.5 nmol/L - 20 nmol/L
2 positive controls, 1 negative control, ready for use
Foetal Type AChR, Adult Type AChR und AChR MAb 2 & 3 Biotin, lyophilized
Enzyme Conjugate 20 x concentrated; Wash buffer 10 x concentrated
All other reagents ready for use
Sample volume: 2 x 50 µl patient serum (no plasma)
1. Sample Preparation: 16-20 hours (over night) incubation at 2-8°C
2. ELISA: 3 hours, incubations at room temperature
Measurement in ELISA reader at 450 nm (570-650 nm reference wavelength)
Neurology, Immunology, Autoimmune Diseases
see Instructions for Use
Negative < 0.45 nmol/L
Positive ≥ 0.45 nmol/LSera from 402 individual healthy blood donors were assayed in the AChRAb ELISA. 401 (99.8%) were identified as being negative for AChRAb.
Sera from 83 patients diagnosed with myasthenia gravis were assayed in the AChRAb ELISA. 76 (92%) were identified as being positive for AChRAb.