Product Information

Acetylcholine Receptor Antibody (ACHRAB® Assay) - ELISA

Catalog-No.

EA110/96
Enzyme Immuno Assay
or the Quantitative Determination of
Acetylcholine Receptor Autoantibodies in Serum

Format

12x8 Determinations,
Microtiter plate, single break apart wells; coated with monoclonal antibody MAb1
4 Standards with range: 0.5 nmol/L - 20 nmol/L
2 positive controls, 1 negative control, ready for use
Foetal Type AChR, Adult Type AChR und AChR MAb 2 & 3 Biotin, lyophilized
Enzyme Conjugate 20 x concentrated; Wash buffer 10 x concentrated
All other reagents ready for use

Procedure

Sample volume: 2 x 50 µl patient serum (no plasma)
1. Sample Preparation: 16-20 hours (over night) incubation at 2-8°C
2. ELISA: 3 hours, incubations at room temperature
Measurement in ELISA reader at 450 nm (570-650 nm reference wavelength)


Clinical Fields

Neurology, Immunology, Autoimmune Diseases

Diseases

Myasthenia gravis

Normal Value

Specificity

Sera from 402 individual healthy blood donors were assayed in the AChRAb ELISA. 401 (99.8%) were identified as being negative for AChRAb.

Sensitivity

Sera from 83 patients diagnosed with myasthenia gravis were assayed in the AChRAb ELISA. 76 (92%) were identified as being positive for AChRAb.